Služby a rezervace

Kontrola kvality

1H NMR is a suitable method for detecting impurities such as solvent residues and it is widely used in the pharmaceutical, medical, and food industries for quality control.

Our laboratory is equipped for measuring liquid and solid samples, and is capable of providing measurements of a wide range of elements. Within the service, we offer measurements of elements 1H, 13C, 15N, 31P, 19F and others with higher resonance frequencies than 15N.

Biomolecular structure and dynamics by NMR

Proteins of up to 200 amino acids, nucleic acids up to 50 nucleotides can be studied. The necessary concentrations are at least 0.1 mM for simple tests, 0.5 mM for more complex studies. Much depends on the molecular weight, folding etc. For solving 3D structures, isotope labeling with ¹⁵N and ¹³C is always needed for proteins and for oligonucleotides larger that approximately 30 nucleotides.

Specialized methods, some of them developed in our laboratory, for charactering inherently disordered proteins (IDPs) are available. We can measure spectra with up to 5 dimensions to assign the protein backbone and sidechain resonances.

NMR pevné fáze

The application of solid-state NMR techniques usually arises due to specific interest in the physics of solid state, including packing effects and polymorphic structures. Solid-state NMR can be also used to study chemical shielding anisotropy. Sometimes, the application is motivated by an inability to dissolve the material of interest.